Show this safety data sheet to the doctor in attendance. Summary a lactophenol cotton blue lpcb stain for detecting eggs of enterobius vermicuaris in perianal surface samples was evaluated prospectively. This stain contains phenol, which will kill the organisms, lactic acid which preserves fungal structures, and cotton blue which stains the chitin found in the fungal cell walls. Lactophenol cotton blue solution material safety data. Once immersed in lactophenol blue solution the organism is rendered safe for handling outside of the biological safety hood for pigmented strains of moulds, lactophenol without cotton blue is available and can be used quality control internal quality control of the lactophenol blue solution must be performed regularly on. Lpcb is a stain used for making semipermanent microscopic preparation of fungi. Ditetesi satu tetes lactophenol cotton blue lpcb pada gelas objek. Staining of fungal cells by using lactophenol cotton blue microbiology with sumi duration. Immediately flush eyes with gentle but large stream of water for at least 15 minutes, lifting lower and upper eyelids occasionally. Summary lactophenol cotton blue stain is formulated with lactophenol, which serves as a mounting fluid, and cotton blue. Fungal cells have both macroscopic as well as microscopic structure. Lactophenol blue solution is readytouse staining solution for fungi staining in bacteriology with samples of human origin. Lactophenol blue solution is a mounting medium and staining agent used in the preparation of slides.
Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database. Use of safe work practices are recommended to avoid eye or skin contact and inhalation of vapours. The stain is recommended for routine use in the wet mount preparation of stools in a parasitology. Phenol 10 29% 108952 water 30 60% 7732185 lactic acid 10 29% 50215 glycerol 10 29% 56815 classified as hazardous according to nohsc criteria synonyms cotton blue, fronine lacto cotton blue. The mount lactophenol cotton blue lpcb was prepared according to. Composition ingredients formula adjusted, standardized to suit performance parameters directions 1 place a drop of lactophenol along with 0. Pdf the use of lactophenol cotton blue lpcb stain for wet mount. Mix the specimen whether a skin scraping, fluid exudate or tissue with two drops of the 10% koh on a. Bd bactec mycof lytic culture vials supplemented middlebrook 7h9 and brain heart infusion broth for use with bd bactec fluorescent series instruments. Pdf evaluation of lactophenol cotton blue for wet mount. Preparation of lactophenol cotton blue slide mounts ncbi. Lactophenol blue solution for microscopy, for staining molds.
Safety data sheet bactidroptm lactophenol aniline blue. Central drug house p ltd 728 vardaan house new delhi2 india. Lactophenol cotton blue stain principle and interpretation. Efficacy of iodineglycerol versus lactophenol cotton blue. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. Not available to individuals or residential addresses. Bd life sciences diagnostic systems package inserts bd. Slide culture technique and lactophenol cotton blue. This readytouse solution is especially for airdried, heatfixed smears of bacteriological material, e. Pour in the lactophenol diluted with methylated spirit until the slides are completely immersed. Lactic acid acts as a preservative and it preserves the structure of fungus. To stain fungal cells by using lactophenol cotton blue. The lactophenol cotton blue lpcb wet mount is most widely used method for the staining and observation of fungi.
Revisions highlighted packing group classification and. Lpcbstained trophozoites and cysts and helminthic ova could easily be detected and identified in lpcb wet mounts of stools. Lactophenol cotton blue wet mount is simple and widelthe y. Our lactophenol blue stain is used as a mounting medium for. Z7 the hardy diagnostics manufacturing facility and quality management system comply with the fdas good manufacturing practices gmp and have. To perform tests, squeeze the dropper to crush the ampule. Lactophenol cotton blue stain fungal element stain for. Organisms suspended in the stain are killed due to the presence of phenol. Lactophenol blue solution lactophenol cotton blue solution. Evaluation of lactophenol cotton blue for wet mount. Lactophenol cotton blue according to regulation ec no 19072006, annex ii, as amended by regulation eu no 4532010 section 1. Here in this mounting method we use lactophenol cotton blue stain where lactophenol acts as a cleaning agent as phenol is fungicidal in nature so it kills the organism. Preparation of lactophenol cotton blue slide mounts.
Jorvet j326b lactophenol cotton blue stain indications. Wet mount preparation is the most widely used method of. Slide culture technique and lactophenol cotton blue staining. Lactophenol is used as a staining solution for wet mounting of fungi. Efficacy of iodineglycerol versus lactophenol cotton blue for. The use of lactophenol cotton blue lpcb stain for wet mount preparation of stools to demonstrate intestinal parasites by routine microscopy was evaluated in this study. Place a drop of lactophenol blue on a clean microscope slide. Pdf the use of lactophenol cotton blue lpcb stain for wet mount preparation of stools to demonstrate intestinal parasites by routine. Others used lactophenol cotton blue 3,5, lactophenoltrypan blue 4, and aniline blue 1. Lactophenol anilin blue solution, lactophenol cotton blue solution. Add a drop of lactophenol cotton blue solution on a slide. License creative commons attribution license reuse allowed.
Add one or two drops of lactophenol cotton blue stain before alcohol gets off. Lactophenol cotton blue used for wet mounts in the examination of yeasts and molds, serving as mounting fluid and stain. Examine under microscope using 10x and 40x objective. The fungal structures include mycelium, sporangiospore, spores etc. Lactophenol cotton blue component classification concentration glycerol. To aid in the microscopic visualization of fungal elements in skin scrapings and hair shafts. Bd stain droppers lactophenol cotton blue life sciences. If the pdf does not display below, you may also download it here. Wear butylneoprene gloves, a type a organic vapour respirator or an airline respirator in confined areas, impervious coveralls and boots.
Warm slowly on a copper stage, covering the dish, but allowing for the escape of vapour. Continue heating for a few minutes after the alcohol has started to vaporise. Page 47 safety data sheet according to whs regulations printing date 29. What safe alternatives are there to lactophenol cotton blue for fungal staining. Lactophenol blue can be used for all the above listed preparations. Fungal structures an overview sciencedirect topics. Lactophenol cotton blue is preferred universally for its usage as a fixative, staining and mounting medium. Evaluation of lactophenol cotton blue for wet mount preparation of feces article pdf available in journal of clinical microbiology 334. Fungi are eukaryotic organism and they are classified into two main groups that is yeast and molds. Chem alert report manufacturers material safety data sheet product name lacto cotton blue emergency spillage if spilt bulk, contact emergency services if appropriate. This item is sold and shipped to schools and businesses only. Invert the dropper for convenient dropbydrop dispensing. The material is stained in one step with lactophenol blue solution.
Start by placing a drop of lactophenol cotton blue reagent on a clean and dry slide. Cool, and transfer the slides to a similar dish of cotton blue in lactophenol, the. Diambil satu pecimen atau bahan pemeriksaan dengan menggunakan ose kemudian diletakkan pada gelas objek tersebut. Lactophenol cotton blue stain definition of lactophenol. The stain is recommended for routine use in the wet mount preparation of stools in a.
Jul 20, 2014 in this prospective study, which was carried out from march to september, 20, we have evaluated the performance of iodineglycerol in comparison to lactophenol cotton blue lpcb and congored formaldehyde as an alternative method for identification of filamentous fungi. Lactophenol cotton blue solution material safety data sheet. Fungus are eukaryotic organism and they are classified into two main groups that is yeast and molds. Jorvet j326b lactophenol cotton blue stain for animal use. I am not very sure what it does in lactophenol cotten blue, but can speculate that it helps in retaining dye cotton blue in the fungal cell wall. Revisions highlighted packing group classification and labelling have been performed according to eu.
Dropper contains oneday supply of reagent or stain, hermetically sealed inside an ampule. Oct 06, 2018 lactophenol cotton blue reagent is used for fungal staining. Lactophenol cotton blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic examination of fungi. Lactophenol along with cotton blue reagent is used for staining as well as for wet mounting of fungi1. Lactophenol cotton blue, 400240, 400241, 400242, 400243 hlk sds. Dualstaining techniques currently in use include safranin and fast green staining flemmer et al. Staining in 5% blue cotton in lactophenol for 20 min macedo, 1997. Product identifier product name lactophenol cotton blue product number pl. Lactophenol cotton blue is widely used to study the. Bactidroptm lactophenol aniline blue safety data sheet date of issue 27092007. Comparison of microscopic morphology of fungi using.
Use of lactophenol cotton blue mounts of corneal scrapings as an. Lactophenol cotton blue, 400240, 400241, 400242, 400243. What safe alternatives are there to lactophenol cotton blue. Aug 17, 2012 short video of using a lactophenol cotton blue stain for identifying fungal elements on a glass slide.
Lactophenol cotton blue lcb is a mixture of methyl blue, a histological stain, and lactophenol a solution of phenol, lactic acid, and glycerol in water. Place the coverslip on the drop avoiding air bubbles to be trapped. In this prospective study, which was carried out from march to september, 20, we have evaluated the performance of iodineglycerol in comparison to lactophenol cotton blue lpcb and congored formaldehyde as an alternative method for identification of filamentous fungi. It is used in wetmount preparations for visualization of fungal structures, especially in medical mycology.
Chem alert report manufacturers material safety data sheet product name lacto cotton blue ingredient conc. Check for and remove contact lenses if present and easy to do. For the staining and microscopic identification of fungi. New staining technique for fungalinfected plant tissues. Lactophenol anilin blue lactophenol cotton blue component classification concentration glycerol cas no. Lactophenol cotton blue reagent is used for fungal staining. Lactophenol cotton blue lpcb is a mounting medium commonly used in microbiology laborato ries, principally for preparing mounts of fungal cul tures. Molds examined in teased mounts must be well teased in order. The lactophenol cotton blue lpcb wet mount preparation is the most widely used method of staining and observing fungi and is simple to prepare. Lactophenol aniline blue may be adequate for presumptive identification, however, colonial morphology and further microscopic examination may be necessary for definitive identification. What safe alternatives are there to lactophenol cotton. Lactophenol cotton blue is a stain that is used to examine fungal elements following either a tape preparation or a scraping. Consult appropriate references for further instructions. Identification of the substancemixture and of the companyundertaking 1.
The slides were analysed, and the images were captured with a leica dc 300f digital camera attached to a leica dmld microscope. Lactophenol blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic examination of fungi. Bd bactec lytic10 anaerobicf culture vials soybeancasein digest broth in a plastic vial. The lactophenol cotton blue wet mount is simple and widely used staining method for fungi. Learn about bd life sciences diagnostic systems package inserts. Staining of fungus by using lactophenol cotton blue.
Lactophenol blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic. Clark et al 2 soaked seeds in lactic acid to soften them and to remove the lemma and palea, afterward staining the seeds with aniline bluelactic acid. Jan 11, 2020 the lactophenol cotton blue lpcb wet mount is most widely used method for the staining and observation of fungi. It is used in wetmount preparations for visualization of fungal structures, especially in medical mycology methyl blue stains fungal cell walls a bright cerulean color, while lactophenol acts as a mountant. For substitution suggestions, please call us at 800. I read some of journal and they used lactophenol cotton blue stain but. Nov 01, 2015 lactophenol cotton blue msds special notice.
917 321 294 454 1245 678 419 1432 510 975 232 1447 911 635 324 474 1115 1131 903 369 275 814 422 153 266 1069 234 237 1483 1162 70 199 194 616 273 719 1258 34 1155 879 1442 1478 357 315 173 575 868